Back to Search
Start Over
Mutations in human lymphocytes studied by an HLA selection system.
- Source :
-
Mutation research [Mutat Res] 1988 Mar; Vol. 198 (1), pp. 221-6. - Publication Year :
- 1988
-
Abstract
- Human lymphocytes mutated at the HLA-A2 or HLA-A3 alleles were enumerated and studied by primary selection using antibody and complement, followed by limiting dilution cloning and secondary selection using immunofluorescence or antibody and complement. The geometric mean frequency of in vivo mutant lymphocytes was 3.08 X 10(-5) for the HLA-A2 allele and 4.68 X 10(-6) for the HLA-A3 allele. Mutagenesis by X-radiation or mitomycin produced a dose-related increase in mutant frequency. HLA-B phenotyping and Southern Analysis of the HLA-A gene suggested that mutation was frequently due to gene deletion, which was often substantial.
Details
- Language :
- English
- ISSN :
- 0027-5107
- Volume :
- 198
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Mutation research
- Publication Type :
- Academic Journal
- Accession number :
- 3258406
- Full Text :
- https://doi.org/10.1016/0027-5107(88)90058-9