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Combining imaging- and gene-based hypoxia biomarkers in cervical cancer improves prediction of chemoradiotherapy failure independent of intratumour heterogeneity.
- Source :
-
EBioMedicine [EBioMedicine] 2020 Jul; Vol. 57, pp. 102841. Date of Electronic Publication: 2020 Jun 21. - Publication Year :
- 2020
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Abstract
- Background: Emerging biomarkers from medical imaging or molecular characterization of tumour biopsies open up for combining the two and exploiting their synergy in treatment planning of cancer patients. We generated a paired data set of imaging- and gene-based hypoxia biomarkers in cervical cancer, appraised the influence of intratumour heterogeneity in patient classification, and investigated the benefit of combining the methodologies in prediction of chemoradiotherapy failure.<br />Methods: Hypoxic fraction from dynamic contrast enhanced (DCE)-MR images and an expression signature of six hypoxia-responsive genes were assessed as imaging- and gene-based biomarker, respectively in 118 patients.<br />Findings: Dichotomous biomarker cutoff to yield similar hypoxia status by imaging and genes was defined in 41 patients, and the association was validated in the remaining 77 patients. The two biomarkers classified 75% of 118 patients with the same hypoxia status, and inconsistent classification was not related to imaging-defined intratumour heterogeneity in hypoxia. Gene-based hypoxia was independent on tumour cell fraction in the biopsies and showed minor heterogeneity across multiple samples in 9 tumours. Combining imaging- and gene-based classification gave a significantly better prediction of PFS than one biomarker alone. A combined dichotomous biomarker optimized in 77 patients showed a large separation in PFS between more and less hypoxic tumours, and separated the remaining 41 patients with different PFS. The combined biomarker showed prognostic value together with tumour stage in multivariate analysis.<br />Interpretation: Combining imaging- and gene-based biomarkers may enable more precise and informative assessment of hypoxia-related chemoradiotherapy resistance in cervical cancer.<br />Funding: Norwegian Cancer Society, South-Eastern Norway Regional Health Authority, and Norwegian Research Council.<br />Competing Interests: Declaration of Competing Interest HL is registered as inventor of a patent application covering the clinical use of the hypoxia gene signature (WO2013/124,738).<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Chemoradiotherapy adverse effects
Diagnostic Imaging
Female
Gene Expression Profiling
Humans
Middle Aged
Norway epidemiology
Prognosis
Progression-Free Survival
Treatment Outcome
Tumor Hypoxia drug effects
Tumor Hypoxia radiation effects
Uterine Cervical Neoplasms diagnostic imaging
Uterine Cervical Neoplasms genetics
Biomarkers, Tumor genetics
Neoplasm Proteins genetics
Uterine Cervical Neoplasms drug therapy
Uterine Cervical Neoplasms radiotherapy
Subjects
Details
- Language :
- English
- ISSN :
- 2352-3964
- Volume :
- 57
- Database :
- MEDLINE
- Journal :
- EBioMedicine
- Publication Type :
- Academic Journal
- Accession number :
- 32580139
- Full Text :
- https://doi.org/10.1016/j.ebiom.2020.102841