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Plasma of Acute Lymphoblastic Leukemia Patients React to the Culture of a Mycovirus Containing Aspergillus flavus.
- Source :
-
Journal of pediatric hematology/oncology [J Pediatr Hematol Oncol] 2020 Jul; Vol. 42 (5), pp. 350-358. - Publication Year :
- 2020
-
Abstract
- Acute lymphoblastic leukemia (ALL) is the most common cancer in children and is also seen in adults. Currently, no plasma-based test for the detection of ALL is available. We have cultured the home of a patient with ALL and isolated a mycovirus containing Aspergillus flavus. This culture was subjected to electron microscopy, purification, and mass spectrometry. Using enzyme-linked immunosorbent assay technique, plasma of patients with ALL and long-term survivors of this disease were tested for antibodies, utilizing supernatant of the culture of this organism. The results were compared with 3 groups of controls, including healthy individuals, patients with sickle cell disease, and solid tumors. Using electron microscopy, the isolated A. flavus contained mycovirus particles. In chemical analysis, this organism did not produce any aflatoxin. Using an enzyme-linked immunosorbent assay technique, the supernatant of the culture of the mycovirus containing A. flavus could differentiate ALL patients from each group of controls (P<0.001). These studies provide a new technique for the detection of ALL and may add information for future research regarding leukemogenesis.
- Subjects :
- Adolescent
Adult
Aspergillosis microbiology
Aspergillosis virology
Case-Control Studies
Child
Child, Preschool
Female
Follow-Up Studies
Humans
Male
Precursor Cell Lymphoblastic Leukemia-Lymphoma blood
Precursor Cell Lymphoblastic Leukemia-Lymphoma etiology
Prognosis
Young Adult
Aspergillosis complications
Aspergillus flavus virology
Fungal Viruses physiology
Plasma microbiology
Precursor Cell Lymphoblastic Leukemia-Lymphoma pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1536-3678
- Volume :
- 42
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of pediatric hematology/oncology
- Publication Type :
- Academic Journal
- Accession number :
- 32576782
- Full Text :
- https://doi.org/10.1097/MPH.0000000000001845