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Tomato SlIDA has a critical role in tomato fertilization by modifying reactive oxygen species homeostasis.

Authors :
Wang R
Shi C
Wang X
Li R
Meng Y
Cheng L
Qi M
Xu T
Li T
Source :
The Plant journal : for cell and molecular biology [Plant J] 2020 Sep; Vol. 103 (6), pp. 2100-2118. Date of Electronic Publication: 2020 Jul 11.
Publication Year :
2020

Abstract

Anther development and pollen tube elongation are key steps for pollination and fertilization. The timing and spatial distribution of reactive oxygen species (ROS) and programmed cell death are central to these processes, but the regulatory mechanism of ROS production is not well understood. Inflorescence deficient in abscission (IDA) is implicated in many plant development and responses to environmental stimuli. However, their role in reproductive development is still unknown. We generated tomato knockout lines (CR-slida) of an IDA homolog (SlIDA), which is expressed in the tapetum, septum and pollen tube, and observed a severe defect in male gametes. Further analysis indicated that there was a programmed cell death defect in the tapetum and septum and a failure of anther dehiscence in the CR-slida lines, likely related to insufficient ROS signal. Liquid chromatography-tandem mass spectrometry identified mature SlIDA as a 14-mer EPIP peptide, which was shown to be secreted, and a complementation experiment showed that application of a synthetic 14-mer EPIP peptide rescued the CR-slida defect and enhanced the ROS signal. Moreover, the application of the ROS scavengers diphenyleneiodonium or Mn-TMPP suppressed peptide function. Collectively, our results revealed that SlIDA plays an essential role in pollen development and pollen tube elongation by modulating ROS homeostasis.<br /> (© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.)

Details

Language :
English
ISSN :
1365-313X
Volume :
103
Issue :
6
Database :
MEDLINE
Journal :
The Plant journal : for cell and molecular biology
Publication Type :
Academic Journal
Accession number :
32573872
Full Text :
https://doi.org/10.1111/tpj.14886