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Structure and expression of genes of GM-CSF and G-CSF in blast cells from patients with acute myeloblastic leukemia.
- Source :
-
Blood [Blood] 1988 Jan; Vol. 71 (1), pp. 204-8. - Publication Year :
- 1988
-
Abstract
- The hematopoietic growth factors granulocyte/macrophage colony-stimulating factor (GM-CSF) and G-CSF, available as recombinant products, stimulate the growth in culture of blasts from patients with acute myeloblastic leukemia (AML). We used cDNA probes for each gene to study the genomic organization in blast cells of 22 patients and expression in the blast cells of 18 patients. Alteration in the structure of G-CSF (two instances) and GM-CSF (two instances) was found. In two patients in whom it was possible to study DNA from bone marrow obtained at remission, the new bands detected in the leukemic cells were not found. Fifteen of 18 patients showed no RNA expression of either growth factor. Both patients with GM-CSF abnormalities as seen by Southern analysis expressed an abnormally large GM-CSF message but no G-CSF messages. One patient with an abnormal Southern pattern with G-CSF expressed normal-sized G-CSF and GM-CSF messages. The biologic significance of these findings remains to be determined. Nonetheless, the abnormal Southern patterns may prove to be useful clonal markers in the study of AML.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Colony-Stimulating Factors biosynthesis
DNA, Neoplasm analysis
Genes
Granulocytes
Humans
Macrophages
Middle Aged
RNA, Messenger analysis
RNA, Neoplasm analysis
Tumor Cells, Cultured analysis
Colony-Stimulating Factors genetics
Leukemia, Myeloid, Acute genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 71
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 3257144