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Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Aug 13; Vol. 529 (1), pp. 112-118. Date of Electronic Publication: 2020 Jun 08. - Publication Year :
- 2020
-
Abstract
- UCH-L1 is a de-ubiquitination enzyme comprehensively distributed in neural cells and podocytes, which is involved in several kinds of nervous system and kidney related diseases. Our previous studies have demonstrated the aberrant up-regulation of UCH-L1 in podocytes of renal diseases, but how dose podocytes are injured by up-regulated UCH-L1 is waiting to be elucidated. Here, we observed the cytoskeleton rearrangement in podocytes with over-expression of UCH-L1, accompanied with a down-regulation of synaptopodin and RhoA, which are closely related to cytoskeletal stabilization. However, we did not see any alteration of RhoA ubiquitination level under the stimulation of UCH-L1 in podocytes. Subsequently, mass spectrum was applied in UCH-L1-flag immunoprecipitation and plakoglobin was screened out, which was among the UCH-L1-combined proteins and most likely related to cytoskeleton rearrangement. Our experiment demonstrates UCH-L1 may not injure podocytes cytoskeleton through a direct regulation on RhoA/Synaptopodin, but through the regulation of plakoglobin, which could be a promising target for treatment of renal disease in the future.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Cytoskeleton metabolism
Cytoskeleton pathology
Humans
Kidney Diseases metabolism
Kidney Diseases pathology
Mice
Microfilament Proteins metabolism
Podocytes pathology
Ubiquitination
gamma Catenin metabolism
rhoA GTP-Binding Protein metabolism
Podocytes metabolism
Ubiquitin Thiolesterase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 529
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 32560811
- Full Text :
- https://doi.org/10.1016/j.bbrc.2020.05.093