Interaction Between NOS3 and HMOX1 on Antihypertensive Drug Responsiveness in Preeclampsia.

Objective:  We examined the interaction of polymorphisms in the genes heme oxygenase-1 ( HMOX1 ) and nitric oxide synthase ( NOS3 ) in patients with preeclampsia (PE) as well as the responsiveness to methyldopa and to total antihypertensive therapy.
Methods:  The genes HMOX1 (rs2071746, A/T) and NOS3 (rs1799983, G/T) were genotyped using TaqMan allele discrimination assays (Applied Biosystems, Foster City, CA, USA ), and the levels of enzyme heme oxygenase-1 ( HO-1) were measured using enzyme-linked immunosorbent assay (ELISA).
Results:  We found interactions between genotypes of the HMOX-1 and NOS3 genes and responsiveness to methyldopa and that PE genotyped as AT presents lower levels of protein HO-1 compared with AA.
Conclusion:  We found interactions between the HMOX-1 and NOS3 genes and responsiveness to methyldopa and that the HMOX1 polymorphism affects the levels of enzyme HO-1 in responsiveness to methyldopa and to total antihypertensive therapy. These data suggest impact of the combination of these two polymorphisms on antihypertensive responsiveness in PE.
Competing Interests: The authors have no conflicts of interest to to declare.
(The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).)