Back to Search
Start Over
Triptolide attenuates laser-induced choroidal neovascularization via M2 macrophage in a mouse model.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2020 Sep; Vol. 129, pp. 110312. Date of Electronic Publication: 2020 Jun 16. - Publication Year :
- 2020
-
Abstract
- Purpose: To investigate whether triptolide has inhibitory effects on the development of choroidal neovascularization (CNV), together with its underlying anti-angiogenic mechanisms.<br />Methods: CNV was induced in C57BL/6 J mice using laser photocoagulation. Triptolide at concentrations of 0.035 and 0.07 mg/kg body weight (BW) or the same volume of phosphate-buffered saline (PBS) was intraperitoneally injected into mice 2 days before laser photocoagulation, which was continued daily till the end of the experiment. CNV areas were measured on day 7. The numbers of M1, M2, and F4/80 <superscript>+</superscript> macrophages were detected on day 1, 3, and 7 in each group. The levels of vascular endothelial growth factor (VEGF) and inflammatory molecules,including intercellular adhesion molecule (ICAM)-1,tumor necrosis factor (TNF)-α, and interleukin 6 (IL-6) were determined by enzyme-linked immunosorbent assay. Cell proliferation, migration, and tube-formation assays were performed in vitro.<br />Results: Triptolide at doses of 0.035 mg/kg BW (66,562 ± 39,253 μm <superscript>2</superscript> , n = 5, P<0.05) and 0.07 mg/kg BW (37,271 ± 25,182 μm <superscript>2</superscript> , n = 5, P<0.001) significantly reduced CNV areas by 54.9 and 74.8 %, respectively, compared with PBS control (147,699 ± 112,900 μm <superscript>2</superscript> , n = 5) in a dose-dependent manner. Protein levels of VEGF, ICAM-1, TNF-α, and IL-6 in the RPE-choroid-sclera complex were significantly downregulated by triptolide treatment on day 3, which was in accordance with the reduced number of infiltrated F4/80 <superscript>+</superscript> macrophages and the reduced ratio of M2/F4/80 <superscript>+</superscript> macrophages. However, no toxic effects of triptolide on the retina or other systemic organs were observed. In addition, triptolide treatment exerted inhibitory effects on cell proliferation, migration, and tube formation in vitro in a concentration-dependent manner.<br />Conclusions: Triptolide has therapeutic potential in CNV owing to its anti-angiogenic effect.<br /> (Copyright © 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Animals
Calcium-Binding Proteins metabolism
Cells, Cultured
Choroidal Neovascularization etiology
Choroidal Neovascularization metabolism
Choroidal Neovascularization pathology
Disease Models, Animal
Dose-Response Relationship, Drug
Epoxy Compounds pharmacology
Human Umbilical Vein Endothelial Cells drug effects
Human Umbilical Vein Endothelial Cells metabolism
Humans
Intercellular Adhesion Molecule-1 metabolism
Interleukin-6 metabolism
Macrophages metabolism
Macrophages pathology
Male
Mice, Inbred C57BL
Phenotype
Receptors, G-Protein-Coupled metabolism
Signal Transduction
Tumor Necrosis Factor-alpha metabolism
Vascular Endothelial Growth Factor A metabolism
Angiogenesis Inhibitors pharmacology
Choroid blood supply
Choroidal Neovascularization prevention & control
Diterpenes pharmacology
Laser Coagulation
Macrophages drug effects
Phenanthrenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 129
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 32559620
- Full Text :
- https://doi.org/10.1016/j.biopha.2020.110312