Streptomyces boluensis sp. nov., isolated from lake sediment.

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain YC537 ^T , was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain YC537 ^T showed that it is closely related to the type strain of Streptomyces ziwulingensis F22 ^T (97.9% sequence similarity), Streptomyces tauricus JCM 4837  ^T (97.7%) and Streptomyces beijiangensis NBRC 100044  ^T (97.6%). The cell wall of the strain contained LL-diaminopimelic acid and the cell-wall sugars were glucose, galactose and ribose. The major phospholipids of strain YC537 ^T were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H ₆ ) and MK-9(H ₈ ). The major cellular fatty acids were iso-C _16:0 , iso-C _14:0 , anteiso-C _15:0 and iso-C _15:0 . Consequently, strain YC537 ^T is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces boluensis sp. nov. is proposed. The type strain is YC537 ^T (= KCTC 39750  ^T  = DSM 102303  ^T ).