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Functional shell matrix proteins tentatively identified by asymmetric snail shell morphology.
- Source :
-
Scientific reports [Sci Rep] 2020 Jun 17; Vol. 10 (1), pp. 9768. Date of Electronic Publication: 2020 Jun 17. - Publication Year :
- 2020
-
Abstract
- Molluscan shell matrix proteins (SMPs) are essential in biomineralization. Here, we identify potentially important SMPs by exploiting the asymmetric shell growth in snail, Lymnaea stagnalis. Asymmetric shells require bilaterally asymmetric expression of SMP genes. We examined expression levels of 35,951 transcripts expressed in the left and right sides of mantle tissue of the pond snail, Lymnaea stagnalis. This transcriptome dataset was used to identify 207 SMPs by LC-MS/MS. 32 of the 207 SMP genes show asymmetric expression patterns, which were further verified for 4 of the 32 SMPs using quantitative PCR analysis. Among asymmetrically expressed SMPs in dextral snails, those that are more highly expressed on the left side than the right side are 3 times more abundant than those that are more highly expressed on the right than the left, suggesting potentially inhibitory roles of SMPs in shell formation. The 32 SMPs thus identified have distinctive features, such as conserved domains and low complexity regions, which may be essential in biomineralization.
- Subjects :
- Animal Shells cytology
Animals
Extracellular Matrix Proteins genetics
Molecular Sequence Annotation
Snails cytology
Snails genetics
Animal Shells metabolism
Extracellular Matrix Proteins metabolism
Functional Laterality genetics
Gene Expression Regulation
Proteome analysis
Snails metabolism
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32555253
- Full Text :
- https://doi.org/10.1038/s41598-020-66021-w