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Dynamic analysis of human small intestinal microbiota after an ingestion of fermented milk by small-intestinal fluid perfusion using an endoscopic retrograde bowel insertion technique.
- Source :
-
Gut microbes [Gut Microbes] 2020 Nov 01; Vol. 11 (6), pp. 1662-1676. Date of Electronic Publication: 2020 Jun 19. - Publication Year :
- 2020
-
Abstract
- Probiotic products have been shown to have beneficial effects on human hosts, but what happens in the gastrointestinal tract after its ingestion remains unclear. Our aim was to investigate the changes within the small intestines after a single intake of a fermented milk product containing a probiotic. We have periodically collected the small-intestinal fluids from the terminal ileum of seven healthy subjects for up to 7 h after ingestion by small-intestinal fluid perfusion using an endoscopic retrograde bowel insertion technique. The bacterial composition of the terminal ileum clearly revealed that the ingested probiotics ( Lactobacillus casei strain Shirota: LcS and Bifidobacterium breve strain Yakult: BbrY) occupied the ileal microbiota for several hours, temporarily representing over 90% of the ileal microbiota in several subjects. Cultivation of ileal fluids showed that under a dramatic pH changes before reaching the terminal ileum, a certain number of the ingested bacteria survived (8.2 ± 6.4% of LcS, 7.8 ± 11.0% of BbrY). This means that more than 1 billion LcS and BbrY cells reached the terminal ileum with their colony-forming ability intact. These results indicate that there is adequate opportunity for the ingested probiotics to continuously stimulate the host cells in the small intestines. Our data suggest that probiotic fermented milk intake affects intestinal microbes and the host, explaining part of the process from the intake of probiotics to the exertion of their beneficial effects on the host.
- Subjects :
- Adult
Animals
Bacteria classification
Bacteria genetics
Bacteria growth & development
Body Fluids diagnostic imaging
Cattle
Cultured Milk Products analysis
Endoscopes
Humans
Intestine, Small diagnostic imaging
Intestine, Small metabolism
Male
Microbial Viability
Middle Aged
Probiotics metabolism
Young Adult
Bacteria isolation & purification
Body Fluids microbiology
Cultured Milk Products microbiology
Gastrointestinal Microbiome
Intestine, Small microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1949-0984
- Volume :
- 11
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Gut microbes
- Publication Type :
- Academic Journal
- Accession number :
- 32552401
- Full Text :
- https://doi.org/10.1080/19490976.2020.1766942