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Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model.
- Source :
-
Science (New York, N.Y.) [Science] 2020 Aug 21; Vol. 369 (6506), pp. 956-963. Date of Electronic Publication: 2020 Jun 15. - Publication Year :
- 2020
-
Abstract
- Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-throughput antibody generation pipeline to rapidly screen more than 1800 antibodies, and established an animal model to test protection. We isolated potent neutralizing antibodies (nAbs) to two epitopes on the receptor binding domain (RBD) and to distinct non-RBD epitopes on the spike (S) protein. As indicated by maintained weight and low lung viral titers in treated animals, the passive transfer of a nAb provides protection against disease in high-dose SARS-CoV-2 challenge in Syrian hamsters. The study suggests a role for nAbs in prophylaxis, and potentially therapy, of COVID-19. The nAbs also define protective epitopes to guide vaccine design.<br /> (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
- Subjects :
- Adult
Angiotensin-Converting Enzyme 2
Animals
Antibody Affinity
Antibody Specificity
Betacoronavirus physiology
Binding Sites
COVID-19
Cell Line
Coronavirus Infections therapy
Coronavirus Infections virology
Disease Models, Animal
Epitopes
Female
Humans
Immunization, Passive
Lung virology
Male
Mesocricetus
Middle Aged
Neutralization Tests
Peptidyl-Dipeptidase A metabolism
Pneumonia, Viral therapy
Pneumonia, Viral virology
Protein Domains
SARS-CoV-2
Spike Glycoprotein, Coronavirus chemistry
Spike Glycoprotein, Coronavirus immunology
Spike Glycoprotein, Coronavirus metabolism
Viral Load
Virus Replication
COVID-19 Serotherapy
Antibodies, Monoclonal blood
Antibodies, Monoclonal immunology
Antibodies, Monoclonal isolation & purification
Antibodies, Monoclonal therapeutic use
Antibodies, Neutralizing blood
Antibodies, Neutralizing immunology
Antibodies, Neutralizing isolation & purification
Antibodies, Neutralizing therapeutic use
Antibodies, Viral blood
Antibodies, Viral immunology
Antibodies, Viral isolation & purification
Antibodies, Viral therapeutic use
Betacoronavirus immunology
Coronavirus Infections immunology
Coronavirus Infections prevention & control
Pandemics prevention & control
Pneumonia, Viral immunology
Pneumonia, Viral prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 369
- Issue :
- 6506
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 32540903
- Full Text :
- https://doi.org/10.1126/science.abc7520