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Nontypeable Haemophilus influenzae Type IV Pilus Mediates Augmented Adherence to Rhinovirus-Infected Human Airway Epithelial Cells.
- Source :
-
Infection and immunity [Infect Immun] 2020 Aug 19; Vol. 88 (9). Date of Electronic Publication: 2020 Aug 19 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Human rhinovirus (hRV) is frequently detected in the upper respiratory tract, and symptomatic infection is associated with an increased nasopharyngeal bacterial load, with subsequent development of secondary bacterial diseases. Nontypeable Haemophilus influenzae (NTHI) is a commensal bacterial species of the human nasopharynx; however, in the context of prior or concurrent upper respiratory tract viral infection, this bacterium commonly causes multiple diseases throughout the upper and lower respiratory tracts. The present study was conducted to determine the mechanism(s) by which hRV infection promotes the development of NTHI-induced diseases. We showed that hRV infection of polarized primary human airway epithelial cells resulted in increased adherence of NTHI, due in part to augmented expression of CEACAM1 and ICAM1, host cell receptors to which NTHI binds via engagement of multiple adhesins. Antibody blockade of these host cell receptors significantly reduced NTHI adherence. With a specific focus on the NTHI type IV pilus (T4P), which we have previously shown binds to ICAM1, an essential adhesin and virulence determinant, we next showed that T4P-directed antibody blockade significantly reduced NTHI adherence to hRV-infected airway cells and, further, that expression of this adhesin was required for the enhanced adherence observed. Collectively, these data provide a mechanism by which "the common cold" promotes diseases due to NTHI, and they add further support for the use of PilA (the majority subunit of T4P) as a vaccine antigen, since antibodies directed against PilA are expected to limit the notably increased bacterial load associated with hRV coinfection and thereby to prevent secondary NTHI-induced diseases of the respiratory tract.<br /> (Copyright © 2020 American Society for Microbiology.)
- Subjects :
- Adhesins, Bacterial genetics
Antibodies, Neutralizing pharmacology
Antigens, CD genetics
Antigens, CD immunology
Cell Adhesion Molecules antagonists & inhibitors
Cell Adhesion Molecules genetics
Cell Adhesion Molecules immunology
Epithelial Cells microbiology
Epithelial Cells virology
Fimbriae Proteins genetics
Gene Expression Regulation immunology
Haemophilus influenzae growth & development
Host-Pathogen Interactions genetics
Humans
Intercellular Adhesion Molecule-1 genetics
Intercellular Adhesion Molecule-1 immunology
Primary Cell Culture
Protein Binding
RNA, Messenger antagonists & inhibitors
RNA, Messenger genetics
RNA, Messenger immunology
Respiratory Mucosa immunology
Respiratory Mucosa microbiology
Respiratory Mucosa virology
Rhinovirus growth & development
Signal Transduction
Adhesins, Bacterial immunology
Bacterial Adhesion immunology
Epithelial Cells immunology
Fimbriae Proteins immunology
Haemophilus influenzae immunology
Host-Pathogen Interactions immunology
Rhinovirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 88
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 32540869
- Full Text :
- https://doi.org/10.1128/IAI.00248-20