Lathyrane diterpenoids from Jatropha podagrica and their antitumor activities in human osteosarcoma cells.

Two new lathyrane-type diterpenoids, jatropodagins A and B ( 1 and 2 ), and five known analogues ( 3 - 7 ), were isolated from the stems of Jatropha podagrica . Their structures and absolute configurations were elucidated by spectroscopic data and calculated ECD analyses. The cytotoxicities of all the lathyrane-type diterpenoids ( 1 - 7 ) were evaluated against two human osteosarcoma cell lines (Saos-2 and MG-63). Compound 1 exhibited significant cytotoxic effects against Saos-2 and MG-63 with IC ₅₀ values of 8.08 and 14.64  μ M, respectively. The IC ₅₀ values for the positive control 5-FU against the Saos-2 and MG-63 cell lines were 19.01 and 25.00  μ M, respectively. Morphological features of apoptosis activities were evaluated in 1 -treated Saos-2 cells and the results confirmed apoptosis in a dose-dependent manner.