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Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2160, pp. 73-81. - Publication Year :
- 2020
-
Abstract
- As one of the essential steps to complete sexual reproduction, a pollen tube is precisely guided to an embryo sac to deliver the sperm cells. This ovule targeting by a pollen tube is one of the essential steps in pollen tube guidance. To assess the ovule targeting ability of the pollen tube from a certain mutant line, comparative analysis of pollen tube behaviors between wild-type and mutant genotypes is important. Here, we provide a protocol that traces all pollen tubes germinated from the quartet tetrad in a pistil by restricted pollination and aniline blue staining. By this analysis, statistical comparison between wild-type and the mutant pollen tube functions under the same in vivo condition is possible.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2160
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 32529429
- Full Text :
- https://doi.org/10.1007/978-1-0716-0672-8_5