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Evaluation of the EDI enzyme linked immunosorbent assays for the detection of SARS-CoV-2 IgM and IgG antibodies in human plasma.
- Source :
-
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2020 Oct; Vol. 509, pp. 79-82. Date of Electronic Publication: 2020 Jun 08. - Publication Year :
- 2020
-
Abstract
- Background: Besides SARS-CoV-2 RT-PCR testing, serological testing is emerging as additional option in COVID-19 diagnostics. Aim of this study was to evaluate novel immunoassays for detection of SARS-CoV-2 antibodies in human plasma.<br />Methods: Using EDI <superscript>TM</superscript> Novel Coronavirus COVID-19 Enzyme Linked Immunosorbent Assays (ELISAs), we measured SARS-CoV-2 IgM and IgG antibodies in 64 SARS-CoV-2 RT-PCR confirmed COVID-19 patients with serial blood samples (n = 104) collected at different time points from symptom onset. Blood samples from 200 healthy blood donors and 256 intensive care unit (ICU) patients collected before the COVID-19 outbreak were also used.<br />Results: The positivity rates in the COVID-19 patients were 5.9% for IgM and 2.9% for IgG ≤ 5 days after symptom onset; Between day 5 and day 10 the positivity rates were 37.1% for IgM and 37.1% for IgG and rose to 76.4% for IgM and 82.4% for IgG after > 10-15 days. After 15-22 days the "true" positivity rates were 94.4% for IgM and 100% for IgG. The "false" positivity rates were 0.5% for IgM and 1.0% for IgG in the healthy blood donors, 1.6% for IgM and 1.2% for IgG in ICU patients.<br />Conclusions: This study shows high "true" vs. low "false" positivity rates for the EDI <superscript>TM</superscript> SARS-CoV-2 IgM and IgG ELISAs.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Aged
Aged, 80 and over
COVID-19
Coronavirus Infections diagnosis
Enzyme-Linked Immunosorbent Assay methods
Enzyme-Linked Immunosorbent Assay standards
Female
Humans
Male
Middle Aged
Pandemics
Pneumonia, Viral diagnosis
SARS-CoV-2
Serologic Tests methods
Antibodies, Viral blood
Betacoronavirus isolation & purification
Coronavirus Infections blood
Immunoglobulin G blood
Immunoglobulin M blood
Pneumonia, Viral blood
Serologic Tests standards
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3492
- Volume :
- 509
- Database :
- MEDLINE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32526218
- Full Text :
- https://doi.org/10.1016/j.cca.2020.05.047