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Tetravalent dengue DNA vaccine is not immunogenic when delivered by retrograde infusion into salivary glands.
- Source :
-
Tropical diseases, travel medicine and vaccines [Trop Dis Travel Med Vaccines] 2020 Jun 03; Vol. 6, pp. 10. Date of Electronic Publication: 2020 Jun 03 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Introduction and Background: A tetravalent DNA vaccine for Dengue virus is under development but has not yet achieved optimal immunogenicity. Salivary glands vaccination has been reported efficacious in rodents and dogs. We report on a pilot study testing the salivary gland as a platform for a Dengue DNA vaccine in a non-human primate model.<br />Materials and Methods: Four cynomolgus macaques were used in this study. Each macaque was pre-medicated with atropine and sedated with ketamine. Stensen's duct papilla was cannulated with a P10 polyethylene tube, linked to a 500ul syringe. On the first two infusions, all macaques were infused with 300ul of TVDV mixed with 2 mg of zinc. For the 3rd infusion, to increase transfection into salivary tissue, two animals received 100uL TVDV mixed with 400uL polyethylenimine 1μg/ml (PEI) and the other two animals received 500uL TVDV with zinc. Antibody titers were assessed 4 weeks following the second and third infusion.<br />Results and Conclusions: SGRI through Stensen's duct is a well-tolerated, simple and easy to reproduce procedure. TVDV infused into macaques salivary glands elicited a significantly weaker antibody response than with different delivery methods.<br />Competing Interests: Competing interestsN/A<br /> (© The Author(s) 2020.)
Details
- Language :
- English
- ISSN :
- 2055-0936
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Tropical diseases, travel medicine and vaccines
- Publication Type :
- Academic Journal
- Accession number :
- 32518668
- Full Text :
- https://doi.org/10.1186/s40794-020-00111-5