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Black Raspberries and Protocatechuic Acid Mitigate DNFB-Induced Contact Hypersensitivity by Down-Regulating Dendritic Cell Activation and Inhibiting Mediators of Effector Responses.
- Source :
-
Nutrients [Nutrients] 2020 Jun 06; Vol. 12 (6). Date of Electronic Publication: 2020 Jun 06. - Publication Year :
- 2020
-
Abstract
- Contact hypersensitivity (CHS) is the most common occupational dermatological disease. Dendritic cells (DCs) mediate the sensitization stage of CHS, while T-cells facilitate the effector mechanisms that drive CHS. Black raspberry ( Rubus occidentalis , BRB) and BRB phytochemicals possess immunomodulatory properties, but their dietary effects on CHS are unknown. We examined the effects of diets containing BRB and protocatechuic acid (PCA, a constituent of BRB and an anthocyanin metabolite produced largely by gut microbes), on CHS, using a model induced by 2,4-dinitrofluorobenze (DNFB). Mice were fed control diet or diets supplemented with BRB or PCA. In vitro bone-marrow derived DCs and RAW264.7 macrophages were treated with BRB extract and PCA. Mice fed BRB or PCA supplemented diets displayed decreased DNFB-induced ear swelling, marked by decreased splenic DC accumulation. BRB extract diminished DC maturation associated with reduced Cd80 expression and Interleukin (IL)-12 secretion, and PCA reduced IL-12. Dietary supplementation with BRB and PCA induced differential decreases in IL-12-driven CHS mediators, including Interferon (IFN)-γ and IL-17 production by T-cells. BRB extracts and PCA directly attenuated CHS-promoting macrophage activity mediated by nitric oxide and IL-12. Our results demonstrate that BRB and PCA mitigate CHS pathology, providing a rationale for CHS alleviation via dietary supplementation with BRB or BRB derived anthocyanins.
- Subjects :
- Animals
B7-1 Antigen metabolism
Dermatitis, Contact etiology
Dermatitis, Contact metabolism
Disease Models, Animal
Interferon-gamma metabolism
Interleukin-12 metabolism
Interleukin-17 metabolism
Mice
Mice, Inbred BALB C
RAW 264.7 Cells
T-Lymphocytes immunology
Dendritic Cells immunology
Dermatitis, Contact immunology
Dermatitis, Contact therapy
Dietary Supplements
Dinitrofluorobenzene adverse effects
Hydroxybenzoates pharmacology
Hydroxybenzoates therapeutic use
Phytotherapy
Plant Extracts pharmacology
Plant Extracts therapeutic use
Rubus
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6643
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Nutrients
- Publication Type :
- Academic Journal
- Accession number :
- 32517233
- Full Text :
- https://doi.org/10.3390/nu12061701