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Optimization of pH Elution Conditions in Immune Complexome Analysis for Comprehensive Identification of Immune Complex Antigens.
- Source :
-
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2020 Nov 10; Vol. 36 (11), pp. 1423-1426. Date of Electronic Publication: 2020 Jun 05. - Publication Year :
- 2020
-
Abstract
- The identification of antigens incorporated into immune complexes (IC-antigens) is important for studying the pathophysiology of immunological diseases. Immune complexome analysis identifies IC-antigens by analyzing ICs collected from biological fluids by IC-capturing beads. In this study, we optimized the method to improve its comprehensiveness while maintaining selectivity for IC-antigens by comparing the number of identified peptides (model IC experiment) or proteins (human pooled serum) eluted from Protein G beads using different pH solutions (pH 2.0 - 11.0).
Details
- Language :
- English
- ISSN :
- 1348-2246
- Volume :
- 36
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32507835
- Full Text :
- https://doi.org/10.2116/analsci.20P112