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A cytokine receptor domeless promotes white spot syndrome virus infection via JAK/STAT signaling pathway in red claw crayfish Cherax quadricarinatus.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2020 Oct; Vol. 111, pp. 103749. Date of Electronic Publication: 2020 Jun 04. - Publication Year :
- 2020
-
Abstract
- The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is pivotal in immune responses for a variety of pathogens in both vertebrates and invertebrates. Domeless (Dome), as a unique cytokine receptor, involves in the upstream JAK/STAT pathway in invertebrates. In this study, the full-length cDNA sequence of a cytokine receptor Dome was identified from red claw crayfish Cherax quadricarinatus (named as CqDome), which contained an open reading frame of 4251 bp, encoding 1416 amino acids. The CqDome contained extracellular conservative domains of a signal peptide, two cytokine binding modules (CBM), three fibronectin-type-III-like (FN3) domains and a transmembrane region. Tissue distribution analysis showed that CqDome generally expressed in all the tissues selected with a high expression in hemocyte. The gene expression of both the viral immediately early gene (IE1) and a late gene envelope protein VP28 of white spot syndrome virus (WSSV) were significantly decreased after gene silencing of CqDome in crayfish haematopoietic tissue (Hpt) cells, indicating a key role of CqDome in promoting WSSV infection. Furthermore, the phosphorylation level of CqSTAT was significantly inhibited by gene silencing of CqDome in Hpt cells, indicating that CqDome participated in signal transduction of JAK/STAT pathway in red claw crayfish. These data together suggest that CqDome is likely to promote WSSV infection via JAK/STAT pathway, which sheds new light on further elucidation of the pathogenesis of WSSV.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Arthropod Proteins genetics
Cells, Cultured
Cloning, Molecular
Drosophila Proteins genetics
Host-Pathogen Interactions
Janus Kinases metabolism
Organ Specificity
Phylogeny
RNA, Small Interfering genetics
Receptors, Interleukin genetics
STAT Transcription Factors metabolism
Signal Transduction
Transcriptome
Arthropod Proteins metabolism
Astacoidea immunology
DNA Virus Infections immunology
Hemocytes physiology
Receptors, Interleukin metabolism
White spot syndrome virus 1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 111
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 32505616
- Full Text :
- https://doi.org/10.1016/j.dci.2020.103749