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Roles for Drosophila cap1 2'-O-ribose methyltransferase in the small RNA silencing pathway associated with Argonaute 2.
- Source :
-
Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2020 Aug; Vol. 123, pp. 103415. Date of Electronic Publication: 2020 Jun 03. - Publication Year :
- 2020
-
Abstract
- Cap1 2'-O-ribose methyltransferase (CMTR1) modifies RNA transcripts containing the 7-methylguanosine cap via 2'-O-ribose methylation of the first transcribed nucleotide, yielding cap1 structures. However, the role of CMTR1 in small RNA-mediated gene silencing remains unknown. Here, we identified and characterized a Drosophila CMTR1 gene (dCMTR1) mutation. We found that the catalytic activity of dCMTR1 was involved in the biogenesis of small interfering RNAs (siRNAs) but not microRNAs. Additionally, dCMTR1 interacted with R2D2, a key component for the assembly of the RNA-induced silencing complex (RISC) containing Argonaute 2 (Ago2). Consistent with this finding, loss of dCMTR1 function impaired RISC assembly by inhibiting the unwinding of Ago2-bound siRNA duplexes, thus preventing the retention of the guide strand. Moreover, dCMTR1 is unlikely to modify siRNAs during RISC assembly. Collectively, our data indicate that dCMTR1 is a positive regulator of the small RNA pathway associated with Ago2 with roles in both siRNA biogenesis and RISC assembly.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Argonaute Proteins metabolism
Drosophila genetics
Drosophila Proteins metabolism
Methylation
MicroRNAs metabolism
Mutation
RNA Interference
Drosophila metabolism
Methyltransferases genetics
Methyltransferases metabolism
RNA, Small Interfering biosynthesis
RNA, Small Interfering chemistry
RNA-Induced Silencing Complex biosynthesis
RNA-Induced Silencing Complex chemistry
RNA-Induced Silencing Complex metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0240
- Volume :
- 123
- Database :
- MEDLINE
- Journal :
- Insect biochemistry and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 32504809
- Full Text :
- https://doi.org/10.1016/j.ibmb.2020.103415