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Design and fabrication of a highly sensitive and naked-eye distinguishable colorimetric biosensor for chloramphenicol detection by using ELISA on nanofibrous membranes.

Authors :
Zhao C
Si Y
Pan B
Taha AY
Pan T
Sun G
Source :
Talanta [Talanta] 2020 Sep 01; Vol. 217, pp. 121054. Date of Electronic Publication: 2020 Apr 18.
Publication Year :
2020

Abstract

Enzyme-linked immunoassay (ELISA) is highly specific and selective towards target molecules and is convenient for on-site detection. However, in many cases, lack of high sensitivity makes it hard to reveal a significant colorimetric signal for detecting a trace amount of target molecules. Thus, analytical instruments are required for detection, which limits the application of ELISA for on-site detection. In the present study, a highly sensitive and naked-eyed detectable colorimetric biosensor for chloramphenicol (CAP) was prepared by incorporating ELISA onto surfaces of microporous and nanofibrous membranes. The high specific surface areas of the nanofibers significantly increased the number of antibodies covalently linked onto the fiber surfaces and binding capacity of the sensor with antigens present in a sample. With such an integration, the sensitivity of the ELISA sensor was dramatically increased, and a trace number of targets could reveal a naked-eye detectable color. The immunoassay sensor exhibited a significant naked-eye distinguishable color to chloramphenicol (CAP) at 0.3 ng/mL. The successful design and fabrication of the nanofibrous membrane immunoassay sensor provide new paths towards the development of on-site inspection sensors without the assistance from any instrument.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3573
Volume :
217
Database :
MEDLINE
Journal :
Talanta
Publication Type :
Academic Journal
Accession number :
32498843
Full Text :
https://doi.org/10.1016/j.talanta.2020.121054