Differential Expression of Bipotent Commitment-Related Genes in Multipotent Mesenchymal Stromal Cells at Different O 2 Levels.

Authors :: Buravkova LB
Ezdakova MI
Andrianova IV
Golikova EA
Andreeva ER
Source :: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2020 Mar; Vol. 491 (1), pp. 67-69. Date of Electronic Publication: 2020 Jun 01.
Publication Year :: 2020
Abstract: The transcriptomic profile associated with osteo- and adipogenic differentiation in growth-arrested multipotent mesenchymal stromal cells (MSCs) from human adipose tissue was analyzed in vitro at 20% (standard laboratory) and 5% (tissue-related) O <subscript>2</subscript> levels. Compared with day 7, at 5% O <subscript>2</subscript> on day 14 spontaneous upregulation of osteo- (RUNX2, SP7, BGLAP, and SPP1) and adipogenic differentiation (CEBPA, PPARG, and ADIPOQ) genes in MSCs was observed (p < 0.05). Thus, upon expansion under tissue-related O <subscript>2</subscript> , MSCs demonstrated a bipotent transcriptomic profile, which may contribute to the improvement of their hematopoiesis-supportive function.

Subjects :: Adipocytes metabolism
Adipogenesis
Adiponectin metabolism
Adipose Tissue metabolism
CCAAT-Enhancer-Binding Proteins metabolism
Cell Differentiation
Cell Hypoxia
Core Binding Factor Alpha 1 Subunit metabolism
Humans
Osteocalcin metabolism
Osteogenesis
Osteopontin metabolism
PPAR gamma metabolism
Sp2 Transcription Factor metabolism
Transcriptome
Gene Expression Profiling
Gene Expression Regulation
Mesenchymal Stem Cells metabolism
Oxygen metabolism

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