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Antibacterial Activity of Fruiting Body Extracts from Culinary-Medicinal Winter Mushroom, Flammulina velutipes (Agaricomycetes) against Oral Pathogen Streptococcus mutans.
- Source :
-
International journal of medicinal mushrooms [Int J Med Mushrooms] 2020; Vol. 22 (2), pp. 115-124. - Publication Year :
- 2020
-
Abstract
- The edible medicinal mushroom Flammulina velutipes (enokitake) has many applications as food and medicine, but its application in dentistry is unknown. This study aims to investigate the inhibitory effect of fruiting body extracts from F. velutipes on the growth and adhesion of Streptococcus mutans, the main cause of human caries, in vitro. Of the four extracts (named TG01 from water, TG02 from 95% ethanol, TG03 from 50% ethanol, and TG04 from ethyl acetate), TG03 had significant antibacterial activity (MIC = 10 mg/mL; MBC = 20 mg/mL). Planktonic growth and biofilm formation in S. mutans was repressed by TG03 at 5 mg/mL and above. Meanwhile, cytotoxicity analysis showed that TG03 was not toxic to human oral keratinocyte cells. HPLC-QQQ-MS analysis showed that the TG03 extract contained a large amount of arabitol, a sucrose substitute that reduces the development of caries. Thus, F. velutipes extracts can effectively inhibit the growth of the oral pathogen S. mutans without cytotoxicity against human oral keratinocytes. Therefore, F. velutipes is a good candidate for the development of oral hygiene agents to control dental caries.
- Subjects :
- Agaricales
Anti-Bacterial Agents isolation & purification
Biofilms growth & development
Cell Adhesion drug effects
Cell Line
Cell Survival drug effects
Chromatography, High Pressure Liquid
Complex Mixtures isolation & purification
Dental Caries microbiology
Fruiting Bodies, Fungal chemistry
Humans
Keratinocytes drug effects
Mass Spectrometry
Microbial Sensitivity Tests
Streptococcus mutans growth & development
Sugar Alcohols isolation & purification
Anti-Bacterial Agents pharmacology
Biofilms drug effects
Complex Mixtures pharmacology
Dental Caries prevention & control
Flammulina chemistry
Streptococcus mutans drug effects
Sugar Alcohols pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-4344
- Volume :
- 22
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of medicinal mushrooms
- Publication Type :
- Academic Journal
- Accession number :
- 32479000
- Full Text :
- https://doi.org/10.1615/IntJMedMushrooms.2020033335