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Impacts of dose and length of exposure to boldenone and stanazolol on enzymatic antioxidant systems, myeloperoxidase and NAGase activities, and glycogen and lactate levels in rat liver.
- Source :
-
Steroids [Steroids] 2020 Sep; Vol. 161, pp. 108670. Date of Electronic Publication: 2020 May 27. - Publication Year :
- 2020
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Abstract
- We investigated the adverse effects of the anabolic androgenic steroids (AAS) boldenone (BOL) and stanazolol (ST) on the enzymatic antioxidant systems of the rat liver. Male Wistar rats were divided in three protocols (P): PI, 5 mg/kg BOL or ST once a week for 4 weeks; PII, 2.5 mg/kg BOL or ST once a week for 8 weeks; PIII, 1.25 mg/kg BOL or ST once a week for 12 weeks. AAS were administered intramuscularly (0.2 ml, olive oil vehicle) once a week in all protocols. Activities of the enzymes glutathione peroxidase (GPx), glutathione S-transferase (GST), and glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), were investigated. We assessed the content of hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ), glycogen and lactate; and enzyme markers of neutrophils (myeloperoxidase, MPO) and macrophages (NAGase). PI and PII altered the SOD and CAT activities and increased the H <subscript>2</subscript> O <subscript>2</subscript> content. PI led to increases in the MPO and NAGase activities. In contrast, changes in GPx, GST and, GR were observed under PII and, to a greater extend, under PIII. Following PIII, GPx, GR, and GST exhibited reduced activities. All protocols altered the glycogen and lactate content. The use of high doses of AAS for a short duration first alters SOD/CAT activity. In contrast, at lower doses of AAS for long periods is associated with changes in the glutathione system. Protocols with high doses of AAS for a short duration exert the most deleterious effects on redox status, markers of cellular infiltration, and the metabolic functioning of hepatic tissues.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Acetylglucosaminidase metabolism
Animals
Dose-Response Relationship, Drug
Liver metabolism
Peroxidase metabolism
Rats
Rats, Wistar
Testosterone pharmacology
Time Factors
Antioxidants metabolism
Glycogen metabolism
Lactic Acid metabolism
Liver drug effects
Liver enzymology
Stanozolol pharmacology
Testosterone analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5867
- Volume :
- 161
- Database :
- MEDLINE
- Journal :
- Steroids
- Publication Type :
- Academic Journal
- Accession number :
- 32473164
- Full Text :
- https://doi.org/10.1016/j.steroids.2020.108670