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Functional inhibition of regulatory CD4+CD25+T cells in peripheral blood of patients with pemphigus vulgaris.
- Source :
-
Clinical and experimental dermatology [Clin Exp Dermatol] 2020 Dec; Vol. 45 (8), pp. 1019-1026. Date of Electronic Publication: 2020 Oct 14. - Publication Year :
- 2020
-
Abstract
- Background: Pemphigus vulgaris (PV) is a potentially life-threatening autoimmune bullous disease affecting the skin and mucous membranes. Its pathogenic mechanism is still not fully understood. Regulatory T cells (Tregs) have been reported to play a significant role in regulating immune homeostasis in autoimmune disorders, such as PV.<br />Aim: To investigate the potential role of Tregs in the immunopathogenesis of PV.<br />Methods: We enrolled 15 patients with PV and 15 healthy controls (HCs). Peripheral blood samples were collected from all participants before treatment. This was followed by flow cytometric, real-time reverse transcription PCR, and in vitro inhibition-based functional assays to explore the immunopathogenesis of Tregs in PV.<br />Results: Our results showed no statistically significant differences in total CD4+CD25+ cells and CD4+CD25 <superscript>high</superscript> cells. In addition, expression levels of FOXP3 mRNA and the corresponding FOXP3 protein remained unchanged in the patients with PV and the HCs. However, the in vitro suppressive activity of CD4+CD25+ T cells was impaired in patients with PV compared with HCs.<br />Conclusions: Our observations suggest that inhibition of suppressive activity of Treg cells may be involved in the pathogenesis of PV.<br /> (© 2020 British Association of Dermatologists.)
- Subjects :
- CD4-Positive T-Lymphocytes metabolism
Case-Control Studies
Flow Cytometry methods
Forkhead Transcription Factors metabolism
Homeostasis immunology
Humans
Interleukin-2 Receptor alpha Subunit metabolism
Pemphigus diagnosis
Pemphigus immunology
Pemphigus pathology
RNA, Messenger genetics
Reverse Transcriptase Polymerase Chain Reaction methods
T-Lymphocytes, Regulatory metabolism
CD4-Positive T-Lymphocytes immunology
Interleukin-2 Receptor alpha Subunit immunology
Pemphigus blood
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2230
- Volume :
- 45
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Clinical and experimental dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 32460351
- Full Text :
- https://doi.org/10.1111/ced.14309