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Antibody-secreting macrophages generated using CpG-free plasmid eliminate tumor cells through antibody-dependent cellular phagocytosis.
- Source :
-
BMB reports [BMB Rep] 2020 Aug; Vol. 53 (8), pp. 442-447. - Publication Year :
- 2020
-
Abstract
- The non-viral delivery of genes into macrophages, known as hard-to-transfect cells, is a challenge. In this study, the microporation of a CpG-free and small plasmid (pCGfd-GFP) showed high transfection efficiency, sustainable transgene expression, and good cell viability in the transfections of Raw 264.7 and primary bone marrow-derived macrophages. The non-viral method using the pCGfd vector encoding anti-EGFR single-chain Fv fused with Fc (scFv-Fc) generated the macrophages secreting anti-EGFR scFv-Fc. These macrophages effectively phagocytized tumor cells expressing EGFR through the antibody-dependent mechanism, as was proved by experiments using EGFR-knockout tumor cells. Finally, peri-tumoral injections of anti-EGFR scFv-Fc-secreting macrophages were shown to inhibit tumor growth in the xenograft mouse model. [BMB Reports 2020; 53(8): 442-447].
- Subjects :
- Animals
Cell Line, Tumor
CpG Islands genetics
ErbB Receptors genetics
ErbB Receptors metabolism
Genes, erbB-1 genetics
Heterografts
Humans
Male
Mice
Mice, Nude
Neoplasms metabolism
Plasmids genetics
RAW 264.7 Cells
Single-Chain Antibodies genetics
Xenograft Model Antitumor Assays
Cytophagocytosis drug effects
Macrophages metabolism
Transfection methods
Subjects
Details
- Language :
- English
- ISSN :
- 1976-670X
- Volume :
- 53
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- BMB reports
- Publication Type :
- Academic Journal
- Accession number :
- 32438971