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Molecular networking uncovers steroidal saponins of Paris tengchongensis.
- Source :
-
Fitoterapia [Fitoterapia] 2020 Sep; Vol. 145, pp. 104629. Date of Electronic Publication: 2020 May 16. - Publication Year :
- 2020
-
Abstract
- Based on a method combining the LC-MS/MS molecular networking strategy with the conventional means of phytochemical research, the chemical constituents and the availability of Paris tengchongensis, a new species found in 2017 from Yunnan Province, were investigated for the first time. The molecular networking showed that this species contained the characteristic steroidal glycosides of the genus Paris by comparison of those of Paris polyphylla var. yunnanensis. Furthermore, the detailed investigation on the 80% EtOH extract of its rhizomes resulted to the isolation of twenty steroidal glycosides including three new spirostane-type saponins, named paristengosides A-C (1-3). Their structures were confirmed by spectroscopic analyses (HRMS and NMR) and chemical methods. The new isolates were evaluated for their cytotoxicities against two human cancer cell lines (HEL and MDA-MB-231), anti-inflammatory effects on a lipopolysaccharide (LPS)-stimulated NO production model in RAW264.7 macrophages, anti-AChE, and antimicrobial activities. The results from the molecular networking and the investigation on the chemical constituents suggested that P. tengchongensis can be used as a potential resource of Rhizoma Paridis.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no financial or non-financial competing interest.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Line, Tumor
China
Cholinesterase Inhibitors isolation & purification
Cholinesterase Inhibitors pharmacology
Chromatography, Liquid
Humans
Mice
Molecular Structure
Nitric Oxide metabolism
Phytochemicals isolation & purification
Phytochemicals pharmacology
Propionibacterium acnes
RAW 264.7 Cells
Saponins isolation & purification
Steroids isolation & purification
Tandem Mass Spectrometry
Melanthiaceae chemistry
Rhizome chemistry
Saponins pharmacology
Steroids pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6971
- Volume :
- 145
- Database :
- MEDLINE
- Journal :
- Fitoterapia
- Publication Type :
- Academic Journal
- Accession number :
- 32428563
- Full Text :
- https://doi.org/10.1016/j.fitote.2020.104629