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Hypoxia regulates human mast cell adhesion to fibronectin via the PI3K/AKT signaling pathway.
- Source :
-
Cell adhesion & migration [Cell Adh Migr] 2020 Dec; Vol. 14 (1), pp. 106-117. - Publication Year :
- 2020
-
Abstract
- A decrease in oxygen concentration is a hallmark of inflammatory reactions resulting from infection or homeostasis disorders. Mast cells interact with extracellular matrix and other cells by adhesion receptors. We investigated the effect of hypoxia on integrin-mediated mast cell adhesion to fibronectin. We found that it was mediated by the α5/β1 receptor and that hypoxia significantly upregulated this process. Hypoxia-mediated increases in mast cell adhesion occurred without increased surface expression of integrins, suggesting regulation by inside-out integrin signaling. Hypoxia also mediated an increase in phosphorylation of Akt, and PI3'kinase inhibitors abolished hypoxia-mediated mast cell adhesion. Hypoxia upregulates the function of integrin receptors by PI3' kinase-dependent signaling. This process might be important for the location of mast cells at inflammatory sites.
- Subjects :
- Cell Adhesion drug effects
Cell Hypoxia drug effects
Cell Line
Humans
Integrin alpha5beta1 metabolism
Mast Cells drug effects
Oligopeptides pharmacology
Phosphatidylinositol 3-Kinases pharmacology
Phosphoinositide-3 Kinase Inhibitors pharmacology
Protein Kinase Inhibitors pharmacology
Receptors, Cell Surface metabolism
Stem Cell Factor pharmacology
Wortmannin pharmacology
Fibronectins pharmacology
Mast Cells cytology
Mast Cells enzymology
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1933-6926
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell adhesion & migration
- Publication Type :
- Academic Journal
- Accession number :
- 32427041
- Full Text :
- https://doi.org/10.1080/19336918.2020.1764690