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Danshen (Salvia miltiorhiza) inhibits Leu27 IGF-II-induced hypertrophy in H9c2 cells.
- Source :
-
Environmental toxicology [Environ Toxicol] 2020 Oct; Vol. 35 (10), pp. 1043-1049. Date of Electronic Publication: 2020 May 16. - Publication Year :
- 2020
-
Abstract
- In this study, we used ICI 182 780 (ICI), an estrogen receptor (ER) antagonist, to investigate the estrogenic activity of Danshen, and to further explored whether Danshen extract can block Leu27IGF-II-induced hypertrophy in H9c2 cardiomyoblast cells. We first used an IGF-II analog Leu27IGF-II, which specifically activates IGF2R signaling cascades and induces H9c2 cardiomyoblast cell hypertrophy. However, Danshen extract completely inhibited Leu27IGF-II-induced cell size increase, ANP and BNP hypertrophic marker expression, and IGF2R induction. We also observed that Danshen extract inhibited calcineurin protein expression and NFAT3 nuclear translocation, leading to suppression of Leu27IGF-II-induced cardiac hypertrophy. Moreover, the anti-Leu27IGF-II-IGF2R signaling effect of Danshen was totally reversed by ICI, which suggest the cardio protective effect of Danshen is mediated through estrogen receptors. Our study suggests that, Danshen exerts estrogenic activity, and thus, it could be used as a selective ER modulator in IGFIIR induced hypertrophy model.<br /> (© 2020 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Calcineurin metabolism
Cardiomegaly prevention & control
Cell Line
Cell Survival drug effects
Drugs, Chinese Herbal isolation & purification
Estrogen Receptor Antagonists pharmacology
Fulvestrant pharmacology
Insulin-Like Growth Factor II pharmacology
Myoblasts, Cardiac metabolism
Myoblasts, Cardiac pathology
Protein Transport
Rats
Receptors, Estrogen metabolism
Signal Transduction
Cell Enlargement drug effects
Drugs, Chinese Herbal pharmacology
Insulin-Like Growth Factor II analogs & derivatives
Myoblasts, Cardiac drug effects
Receptor, IGF Type 2 metabolism
Salvia miltiorrhiza chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1522-7278
- Volume :
- 35
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Environmental toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 32415908
- Full Text :
- https://doi.org/10.1002/tox.22940