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Establishment of a longitudinal pre-clinical model of lyssavirus infection.
- Source :
-
Journal of virological methods [J Virol Methods] 2020 Jul; Vol. 281, pp. 113882. Date of Electronic Publication: 2020 May 12. - Publication Year :
- 2020
-
Abstract
- Traditional mouse models of lyssavirus pathogenesis rely on euthanizing large groups of animals at various time points post-infection, processing infected tissues, and performing histological and molecular analyses to determine anatomical sites of infection. While powerful by some measures, this approach is limited by the inability to monitor disease progression in the same mice over time. In this study, we established a novel non-invasive mouse model of lyssavirus pathogenesis, which consists of longitudinal imaging of a luciferase-expressing Australian bat lyssavirus (ABLV) reporter virus. In vivo bioluminescence imaging (BLI) in mice revealed viral spread from a peripheral site of inoculation into the central nervous system (CNS), with kinetically and spatially distinct foci of replication in the footpad, spinal cord, and hindbrain. Detection of virus within the CNS was associated with onset of clinical disease. Quantification of virus-derived luminescent signal in the brain was found to be a reliable measure of viral replication, when compared to traditional molecular methods. Furthermore, we demonstrate that in vivo imaging of ABLV infection is not restricted to the use of albino strains of mice, but rather strong BLI signal output can be achieved by shaving the hair from the heads and spines of pigmented strains, such as C57BL/6. Overall, our data show that in vivo BLI can be used to rapidly and non-invasively identify sites of lyssavirus replication and to semi-quantitatively determine viral load without the need to sacrifice mice at multiple time points.<br />Competing Interests: Declaration of Competing Interest None.<br /> (Published by Elsevier B.V.)
- Subjects :
- Animals
Brain virology
Cell Line
Female
HEK293 Cells
Humans
Longitudinal Studies
Luciferases genetics
Luminescent Measurements
Lyssavirus enzymology
Lyssavirus genetics
Male
Mice
Mice, Inbred C57BL
Molecular Imaging
Rhabdoviridae Infections immunology
Viral Load
Antibodies, Viral blood
Disease Models, Animal
Lyssavirus pathogenicity
Rhabdoviridae Infections virology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0984
- Volume :
- 281
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 32407866
- Full Text :
- https://doi.org/10.1016/j.jviromet.2020.113882