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CaMKII is activated in opioid induced conditioned place preference, but αCaMKII Thr286 autophosphorylation is not necessary for its establishment.
- Source :
-
Behavioural brain research [Behav Brain Res] 2020 Jul 15; Vol. 390, pp. 112676. Date of Electronic Publication: 2020 May 12. - Publication Year :
- 2020
-
Abstract
- Activation of calcium/calmodulin-dependent protein kinase II (CaMKII), particularly its α isoform, is known to be important for neuronal processes central for learning and memory and has also been implicated in the maladaptive learning involved in drug addiction.Thr286 autophosphorylation of αCaMKII has been shown to be indispensable for establishment of cocaine-induced CPP (Easton et al., 2014). To study the contribution of CaMKII in opioid induced conditioned learning, we examined how establishment of conditioned place preference (CPP) induced by 10 or 30 μmol/kg morphine or its active metabolite morphine-6-glucuronide (M6G) affects the levels and Thr286 autophosphorylation of the α- and β-isoforms of CaMKII, as well as β-actin levels, in dorsal and ventral striatum and in hippocampus of mice. An acute and a sub-chronic treatment were used as controls. Whereas an acute single administration of morphine or M6G caused increases in CaMKII levels and phosphorylation at Thr286 and β-actin in striatal areas, CPP induced by these opioids was accompanied primarily by an increase in the protein levels of both CaMKII isoforms and β-actin in dorsal striatum and hippocampus. Decreases in CaMKII Thr286 phosphorylation were observed in dorsal striatum after the sub-chronic pharmacological treatment. Despite the changes observed in αCaMKII activity in wild type mice, morphine-induced CPP was not affected in αCaMKII <superscript>T286A</superscript> autophosphorylation-deficient mice. These results indicate that opioid-induced CPP is accompanied by activation of α- and βCaMKII in striatum and hippocampus, but, in opposition to what has been observed with cocaine, αCaMKII autophosphorylation is not essential for establishment of opioid-induced CPP.<br />Competing Interests: Declaration of Competing Interest None.<br /> (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Analgesics, Opioid administration & dosage
Animals
Behavior, Animal drug effects
Behavior, Animal physiology
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Morphine pharmacology
Morphine Derivatives pharmacology
Phosphorylation
Actins drug effects
Actins metabolism
Analgesics, Opioid pharmacology
Calcium-Calmodulin-Dependent Protein Kinase Type 2 drug effects
Calcium-Calmodulin-Dependent Protein Kinase Type 2 metabolism
Conditioning, Classical drug effects
Conditioning, Classical physiology
Hippocampus drug effects
Hippocampus metabolism
Neostriatum drug effects
Neostriatum metabolism
Neuronal Plasticity drug effects
Neuronal Plasticity physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7549
- Volume :
- 390
- Database :
- MEDLINE
- Journal :
- Behavioural brain research
- Publication Type :
- Academic Journal
- Accession number :
- 32407818
- Full Text :
- https://doi.org/10.1016/j.bbr.2020.112676