Back to Search
Start Over
Validated LC-MS/MS Method for Simultaneous Quantitation of SAFit-1 and SAFit-2 in Mice Plasma: Application to a Pharmacokinetic Study.
- Source :
-
Drug research [Drug Res (Stuttg)] 2020 Jul; Vol. 70 (7), pp. 325-332. Date of Electronic Publication: 2020 May 13. - Publication Year :
- 2020
-
Abstract
- SAFit-1 and SAFit-2 are selective FKBP51 (FK506-binding protein 51) ligands. In this paper, we present the development and validation data of an LC-MS/MS method for the simultaneous quantitation of SAFit-1 and SAFit-2 in mice plasma as per FDA regulatory guideline. SAFit-1 and SAFit-2 along with internal standard were extracted from mice plasma using liquid-liquid extraction method. Chromatographic resolution of SAFit-1, SAFit-2 and the internal standard (warfarin) was achieved on an X-Terra phenyl column using 0.2% formic acid:acetonitrile (20:80, v/v) as an eluent, which was delivered at a flow-rate of 0.9 mL/min. The MS/MS ion transitions monitored were m/z 748.4→420.4, 803.7→384.3 and 309.2 →163.2 for SAFit-1, SAFit-2 and the internal standard, respectively. The linearity range was 2.45-2446 ng/mL for both SAFit-1 and SAFit-2. The intra- and inter-day accuracy and intra- and inter-day precision were in the range of 0.90-1.07 and 2.38-10.8%, respectively for SAFit-1; 0.97-1.15 and 0.23-12.5%, respectively for SAFit-2. Both SAFit-1 and SAFit-2 were found to be stable in stability studies (up to three freeze-thaw cycles and for long-term at -80°C for 30 days) and processed (bench-top for 3 h and in in-injector for 16 h) samples. The application of the validated method was shown in a pharmacokinetic study in mice.<br />Competing Interests: The authors declare that they have no conflict of interest.<br /> (© Georg Thieme Verlag KG Stuttgart · New York.)
- Subjects :
- Administration, Oral
Animals
Antidepressive Agents administration & dosage
Antidepressive Agents pharmacokinetics
Chromatography, High Pressure Liquid
Male
Mice
Sensitivity and Specificity
Tandem Mass Spectrometry
Antidepressive Agents analysis
Chemistry, Pharmaceutical methods
Tacrolimus Binding Proteins antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 2194-9387
- Volume :
- 70
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Drug research
- Publication Type :
- Academic Journal
- Accession number :
- 32403135
- Full Text :
- https://doi.org/10.1055/a-1164-6123