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Modulation of circRNA Metabolism by m 6 A Modification.
- Source :
-
Cell reports [Cell Rep] 2020 May 12; Vol. 31 (6), pp. 107641. - Publication Year :
- 2020
-
Abstract
- N <superscript>6</superscript> -methyladenosine (m <superscript>6</superscript> A) is an RNA modification well-known for its contribution to different processes controlling RNA metabolism, including splicing, stability, and translation of mRNA. Conversely, the role of m <superscript>6</superscript> A on the biogenesis and function of circular RNAs (circRNAs) has yet to be addressed. circRNAs belong to a class of covalently closed transcripts produced via a back-splicing reaction whereby a downstream 5' splice donor site fuses to an upstream 3' splice acceptor site. Starting from circ-ZNF609 as a study case, we discover that specific m <superscript>6</superscript> As control its accumulation and that METTL3 and YTHDC1 are required to direct the back-splicing reaction. This feature is shared with other circRNAs because we find a significant direct correlation among METTL3 requirement, YTHDC1 binding, and the ability of m <superscript>6</superscript> A exons to undergo back-splicing. Finally, because circ-ZNF609 displays the ability to be translated, we show that m <superscript>6</superscript> A modifications, through recognition by YTHDF3 and eIF4G2, modulate its translation.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.<br /> (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 31
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 32402287
- Full Text :
- https://doi.org/10.1016/j.celrep.2020.107641