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Smart drug delivery against Helicobacter pylori: pectin-coated, mucoadhesive liposomes with antiadhesive activity and antibiotic cargo.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2020 Jul; Vol. 104 (13), pp. 5943-5957. Date of Electronic Publication: 2020 May 12. - Publication Year :
- 2020
-
Abstract
- The first step in the development of Helicobacter pylori pathogenicity is the receptor-mediated adhesion to the gastric epithelium. Inhibition of outer membrane proteins of H. pylori (e.g. BabA) by antiadhesive drugs will contribute to reduced recolonization and infection. Pectin from apple inhibits the BabA and LPS-mediated adhesion of H. pylori to human stomach cells. Pectin-coated liposomes with encapsulated amoxicillin were characterized for polydispersity, zeta potential, encapsulation efficiency, stability, and amoxicillin release. Coated liposomes did not influence the viability of AGS and HT29-MTX cells up to 100 μg/mL but exert cytotoxicity against H. pylori at 10 μg/mL. Pectin-coating of liposomes provoked direct interaction and subsequent binding of the particles to surface structures of H. pylori, and interaction with mucus from porcine stomach and mucus secreted by HT29-MTX cells. Laser scanning microscopy of H. pylori and AGS cells together with liposomes indicated co-aggregation. The mucoadhesive effect seems interesting as stomach cells are covered by a mucus layer. H. pylori is able to penetrate and cross the mucin rapidly to reach pH-neutral epithelium to escape the acidic environment, followed by interaction with epithelial cells. In summary, all experimental evidence is consistent with a specific interaction of pectin-coated liposomes with mucins and surface structures of H. pylori. As the coated liposomes show mucoadhesion to the negatively charged mucins, docking to stomach mucin, mucus penetration, and recognition of and adhesion to H. pylori, they can be considered a novel type of multifunctional drug carriers for local antibiotic therapy against H. pylori. KEY POINTS: • Smart, multifunctional mucoadhesive liposomes • Specific targeting against BabA/LPS of Helicobacter pylori • Inhibition of bacterial adhesion of H. pylori to human host cells • Release of antibiotic cargo.
- Subjects :
- Adhesins, Bacterial metabolism
Amoxicillin chemistry
Amoxicillin pharmacology
Animals
Anti-Bacterial Agents chemistry
Anti-Bacterial Agents metabolism
Cell Line
Gastric Mucins metabolism
Gastric Mucosa metabolism
Gastric Mucosa microbiology
Helicobacter pylori metabolism
Humans
Lipopolysaccharides metabolism
Liposomes metabolism
Swine
Anti-Bacterial Agents pharmacology
Bacterial Adhesion drug effects
Drug Delivery Systems
Helicobacter pylori drug effects
Liposomes chemistry
Pectins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 104
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 32399588
- Full Text :
- https://doi.org/10.1007/s00253-020-10647-3