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CD44+/EPCAM+ cells detect a subpopulation of ALDH high cells in human non-small cell lung cancer: A chance for targeting cancer stem cells?
- Source :
-
Oncotarget [Oncotarget] 2020 Apr 28; Vol. 11 (17), pp. 1545-1555. Date of Electronic Publication: 2020 Apr 28 (Print Publication: 2020). - Publication Year :
- 2020
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Abstract
- Objectives: Several studies demonstrated that aldehyde dehydrogenase (ALDH) and CD44 are the most considered cancer stem cells (CSC) markers. However, a comparison between ALDH high cells and CD44+ cells have been previously described with no significant correlation. Indeed, the aim of the present research is to identify a superficial marker able to match with ALDH high cells population in freshly isolated human lung cancer cells.<br />Materials and Methods: This cross-sectional study analyzed the expression of ALDH <superscript>high/low</superscript> cells and the positivity for CD44 and epithelium cell adhesion molecule (EPCAM) antigens in surgical lung cancer tissues. The main approach was a cytofluorimetric analysis of ALDH expression and positivity for CD44/EPCAM on primary cell population obtained from 23 patients harboring NSCLC.<br />Results: There was a highly positive correlation between the expressions of ALDH <superscript>high</superscript> and CD44+/EPCAM+ cells, with a Pearson's correlation coefficient equal to 0.69 (95% CI 0.39-0.86; P = 0.0002), and Spearman's correlation coefficient equal to 0.52 ( P = 0.0124). The average paired difference between the expression of ALDH <superscript>high</superscript> and CD44+/EPCAM+ cells was very close to 0, being 0.1% (SD 2.5%); there was no difference between these subpopulations in terms of means (95% CI = -1.0; 1.2%, P = 0.8464). These results highlight a strong similarity between ALDH <superscript>high</superscript> and CD44+/EPCAM+ cells.<br />Conclusions: Our study is the first attempt which identifies a high correlation between the ALDH <superscript>high</superscript> and the CD44+/EPCAM+ cells, thus suggesting the possibility to use this superficial marker for future target treatments against lung cancer stem cells.<br />Competing Interests: CONFLICTS OF INTEREST None of the Authors have any competing interests.
Details
- Language :
- English
- ISSN :
- 1949-2553
- Volume :
- 11
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Oncotarget
- Publication Type :
- Academic Journal
- Accession number :
- 32391123
- Full Text :
- https://doi.org/10.18632/oncotarget.27568