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Bioactive antibacterial bilayer PCL/gelatin nanofibrous scaffold promotes full-thickness wound healing.
- Source :
-
International journal of pharmaceutics [Int J Pharm] 2020 Jun 15; Vol. 583, pp. 119413. Date of Electronic Publication: 2020 May 07. - Publication Year :
- 2020
-
Abstract
- Treatment of diabetic, chronic, and full-thickness wounds is a challenge as these injuries usually lead to infections that cause delayed and inappropriate healing. Therefore, fabrication of skin scaffolds with prolonged antibacterial properties are of great interest. Due to this demand, bilayered nanofibrous scaffolds were fabricated based on polycaprolactone and gelatin. The top layer of these scaffolds contained amoxicillin as a model drug and the bottom layer was loaded with zinc oxide nanoparticles to accelerate wound healing. Several characterization techniques including FTIR, SEM, swelling, tensile test, in vitro degradation, drug release, antibacterial activity, and MTT assay were used to assess physical, mechanical, and biological properties of produced nanofibers. SEM results demonstrated that bilayered scaffolds have smooth bead-free microstructures while in vitro release test showed that samples have a sustained release for amoxicillin up to 144 h (tested time). Disk diffusion assessment confirmed the potency of scaffolds for hindering bacterial growth while results of cytotoxicity evaluation revealed that scaffolds could effectively accelerate cell proliferation. Finally, in vivo tests on full-thickness rat models revealed that fabricated nanofibers accelerate wound contraction, increase collagen deposition and angiogenesis, and prevent scar formation. Altogether, results showed that fabricated scaffolds are promising candidates for treatment of full-thickness wounds.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Amoxicillin chemistry
Animals
Anti-Bacterial Agents chemistry
Cell Proliferation drug effects
Delayed-Action Preparations
Disease Models, Animal
Drug Compounding
Drug Liberation
Drug Stability
Male
Neovascularization, Physiologic drug effects
Rats, Sprague-Dawley
Skin injuries
Tensile Strength
Wounds and Injuries pathology
Zinc Oxide chemistry
Amoxicillin administration & dosage
Anti-Bacterial Agents administration & dosage
Drug Carriers
Gelatin chemistry
Nanofibers
Polyesters chemistry
Skin blood supply
Skin drug effects
Wound Healing drug effects
Wounds and Injuries drug therapy
Zinc Oxide administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3476
- Volume :
- 583
- Database :
- MEDLINE
- Journal :
- International journal of pharmaceutics
- Publication Type :
- Academic Journal
- Accession number :
- 32389791
- Full Text :
- https://doi.org/10.1016/j.ijpharm.2020.119413