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Synthesis and Evaluation of New Trivalent Ligands for Hepatocyte Targeting via the Asialoglycoprotein Receptor.
- Source :
-
Bioconjugate chemistry [Bioconjug Chem] 2020 May 20; Vol. 31 (5), pp. 1313-1319. Date of Electronic Publication: 2020 May 09. - Publication Year :
- 2020
-
Abstract
- Since the asialoglycoprotein receptor (also known as the "Ashwell-Morell receptor" or ASGPR) was discovered as the first cellular mammalian lectin, numerous drug delivery systems have been developed and several gene delivery systems associated with multivalent ligands for liver disease targeting are undergoing clinical trials. The success of these systems has facilitated the further study of new ligands with comparable or higher affinity and less synthetic complexity. Herein, we designed two novel trivalent ligands based on the esterification of tris(hydroxymethyl) aminomethane (TRIS) followed by the azide-alkyne Huisgen cycloaddition with azido N -acetyl-d-galactosamine. The presented triazolyl glycoconjugates exhibited good binding to ASGPR, which was predicted using in silico molecular docking and assessed by a surface plasmon resonance (SPR) technique. Moreover, we demonstrated the low level of in vitro cytotoxicity, as well as the optimal spatial geometry and the required amphiphilic balance, for new, easily accessible ligands. The conjugate of a new ligand with Cy5 dye exhibited selective penetration into HepG2 cells in contrast to the ASGPR-negative PC3 cell line.
- Subjects :
- Alkynes chemistry
Asialoglycoprotein Receptor chemistry
Azides
Chemistry Techniques, Synthetic
Drug Design
Esterification
Galactosamine chemistry
Hep G2 Cells
Humans
Ligands
Methane chemical synthesis
Methane chemistry
Methane metabolism
Methane pharmacology
Molecular Docking Simulation
PC-3 Cells
Protein Conformation
Asialoglycoprotein Receptor metabolism
Hepatocytes drug effects
Hepatocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-4812
- Volume :
- 31
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Bioconjugate chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32379426
- Full Text :
- https://doi.org/10.1021/acs.bioconjchem.0c00202