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Genome constellations of rotavirus a isolated from avian species in Brazil, 2008-2015.
- Source :
-
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2020 Sep; Vol. 51 (3), pp. 1363-1375. Date of Electronic Publication: 2020 May 06. - Publication Year :
- 2020
-
Abstract
- Rotaviruses are members of the family Reoviridae and are a common cause of acute diarrhea in many mammalian and avian species. They are non-enveloped icosahedral particles and their genome comprises 11 segments of double-stranded RNA, which encodes six structural proteins (VP1-4, VP6-7) and six nonstructural proteins (NSP1-6). Genotypes are defined based upon the diversity found in these genes and viral characterization plays a central role on epidemiological studies and prevention. Here we investigate the distribution of Brazilian RVAs genotypes in 8 chicken samples collected between 2008 and 2015 from different regions by RT-PCR, partial (Sanger) nucleotide sequencing and phylogenetic analysis from all rotavirus genes. Although the identified genotypes were typical from avian host species, when analyzed together, they form novel genetic constellations: G19-P[31]-I11-R6-C6-M7-A16-N6-T8-E10-H8 and G19-P[31]-I4-R4-C4-M4-A16-N4-T4-E4-H4. This study highlights that avian rotaviruses are widespread among commercial farms in Brazil, and the co-circulation of at least two different genomic constellations indicates that may present a way bigger genetic variability, that can be increased by the possible transmission events from other birds, lack of specific preventive measures, as well as the different viral evolution mechanisms.
- Subjects :
- Animals
Base Sequence
Brazil
Chickens
Genetic Variation
Genotype
Phylogeny
RNA, Viral genetics
Rotavirus classification
Rotavirus isolation & purification
Rotavirus Infections virology
Bird Diseases virology
Genome, Viral
Poultry Diseases virology
Rotavirus genetics
Rotavirus Infections veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1678-4405
- Volume :
- 51
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
- Publication Type :
- Academic Journal
- Accession number :
- 32378061
- Full Text :
- https://doi.org/10.1007/s42770-020-00259-4