Antimicrobial and Antioxidant Effects of a Forest Actinobacterium V 002 as New Producer of Spectinabilin, Undecylprodigiosin and Metacycloprodigiosin.

The aim of the study is the research and identification of a Streptomyces strain as a new producer of spectinabilin, undecylprodigiosin and metacycloprodigiosin. Among 54 actinomycete isolates isolated from El-Ogbane forest soils in Algeria, only one isolate, designated V ₀₀₂ , was selected for its ability to produce prodigiosins. The selected strain was analysed for its ability to produce three different secondary metabolites as well as their biological activities. V ₀₀₂ belongs to the Streptomyces genus and has significant antimicrobial and antioxidant activities. The taxonomic position of V ₀₀₂ by 16S rRNA sequence analysis showed a similarity of 99.93% with Streptomyces lasiicapitis DSM 103124 ^T and 98.96% with Streptomyces spectabilis DSM 40512 ^T . Fractionation of crude secondary metabolites produced by the strain using HPLC-MS revealed the presence of spectinabilin, undecylprodigiosin and metacycloprodigiosin, which demonstrated significant activity. Strain V ₀₀₂ is considered a new producer of spectinabilin, undecylprodigiosin and metacycloprodigiosin with significant antimicrobial and antioxidant activity.