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The role of Piper chaba Hunt. and its pure compound, piperine, on TRPV1 activation and adjuvant effect.
- Source :
-
BMC complementary medicine and therapies [BMC Complement Med Ther] 2020 May 05; Vol. 20 (1), pp. 134. Date of Electronic Publication: 2020 May 05. - Publication Year :
- 2020
-
Abstract
- Background: Piper chaba Hunt. is used as an ingredient in Thai traditional preparation for arthritis. Its isolated compound is piperine which shows anti-inflammatory activity. Piperine produces a burning sensation because it activates TRPV1 receptor. The TRPV1 activation involved with the analgesic and adjuvant effect. P. chaba Hunt. has not been reported about TRPV1 activation and adjuvant effect. The aim of this study was to investigate the effect of P. chaba extract and piperine on TRPV1 receptor, which is considered as a target for analgesic and their adjuvant effects to support the development of an analgesic drug from herbal medicine.<br />Methods: The effect of P. chaba extract and piperine on HEK cells expressing TRPV1 channel was examined by calcium imaging assay. Adjuvant effects of P. chaba extract and piperine were investigated by a fluorescein isothiocyanate (FITC)-induced contact hypersensitivity (CHS) model in mice.<br />Results: P. chaba extract induced calcium influx with EC <subscript>50</subscript> value of 0.67 μg/ml. Piperine induced calcium influx with EC <subscript>50</subscript> value of 0.31 μg/ml or 1.08 μM. For mouse CHS model, we found that 1% piperine, 5% piperine, 1% P. chaba extract and 5% P. chaba extract significantly enhanced sensitization to FITC as revealed by ear swelling responses.<br />Conclusion: P. chaba extract and piperine activated TRPV1 channel and enhanced contact sensitization to FITC.
- Subjects :
- Adjuvants, Pharmaceutic chemistry
Alkaloids chemistry
Animals
Benzodioxoles chemistry
HEK293 Cells
Humans
Mice
Mice, Inbred BALB C
Piperidines chemistry
Plant Extracts chemistry
Polyunsaturated Alkamides chemistry
Thailand
Adjuvants, Pharmaceutic pharmacology
Alkaloids pharmacology
Benzodioxoles pharmacology
Piper
Piperidines pharmacology
Plant Extracts pharmacology
Polyunsaturated Alkamides pharmacology
TRPV Cation Channels metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2662-7671
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC complementary medicine and therapies
- Publication Type :
- Academic Journal
- Accession number :
- 32370771
- Full Text :
- https://doi.org/10.1186/s12906-020-02917-4