Back to Search
Start Over
Detection of Zika Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP).
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2142, pp. 137-146. - Publication Year :
- 2020
-
Abstract
- Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) allows amplification and detection of RNA or DNA rapidly and relatively inexpensively. Here we describe how RT-LAMP can be utilized to detect Zika virus in human urine or serum samples or Aedes mosquito samples. This can be completed in under 30 min and without first isolating the RNA from the sample.
- Subjects :
- Aedes virology
Animals
Humans
Limit of Detection
Point-of-Care Testing
RNA, Viral genetics
RNA, Viral isolation & purification
Sensitivity and Specificity
Serologic Tests methods
Urinalysis methods
Zika Virus Infection blood
Zika Virus Infection urine
Molecular Diagnostic Techniques methods
Nucleic Acid Amplification Techniques methods
Zika Virus genetics
Zika Virus isolation & purification
Zika Virus Infection diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2142
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 32367365
- Full Text :
- https://doi.org/10.1007/978-1-0716-0581-3_12