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Co-administration of Erythromycin and Leech Salivary Extract Alleviates Osteomyelitis in Rats Induced by Methicillin-Resistant Staphylococcus aureus.
Co-administration of Erythromycin and Leech Salivary Extract Alleviates Osteomyelitis in Rats Induced by Methicillin-Resistant Staphylococcus aureus.
- Source :
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Veterinary and comparative orthopaedics and traumatology : V.C.O.T [Vet Comp Orthop Traumatol] 2020 Jul; Vol. 33 (4), pp. 243-251. Date of Electronic Publication: 2020 Apr 30. - Publication Year :
- 2020
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Abstract
- Objective: Erythromycin (Ery) and leech saliva (LS) can inhibit Staphylococcus aureus growth in in vitro conditions. This study aimed to evaluate the activities and synergy between Ery and LS on chronic osteomyelitis in male Wistar rat's tibia induced by methicillin-resistant S. aureus (MRSA).<br />Materials and Methods: Four weeks after osteomyelitis induction, rats were divided into four groups including no treatment (control), Ery monotherapy (orally), LS monotherapy, or Ery + LS twice daily for 2 weeks. Staphylococcus aureus growth, pathological signs and inflammatory cytokine tumour necrosis factor-alpha (TNF-α) levels were assessed.<br />Results: Rats tolerated all therapeutic strategies well during the experiment. The Ery treatment alone significantly decreased bacterial growth, pathological signs and TNF-α levels. Leech saliva alone reduced TNF-α level significantly, but did not produce a significant reduction in bacterial growth and pathological signs. Ery + LS treatment significantly decreased bacterial growth, considerably alleviated bone pathological signs and decreased TNF-α levels compared with other groups. Statistical analysis suggested that there was a stronger efficiency and synergistic action of Ery and LS when combined against MRSA-induced osteomyelitis in rats.<br />Clinical Significance: The present study suggests that LS may have clinical utility to treat MRSA-induced osteomyelitis when combined with Ery or other therapeutics.<br />Competing Interests: None declared.<br /> (Georg Thieme Verlag KG Stuttgart · New York.)
- Subjects :
- Animals
Male
Osteomyelitis diagnostic imaging
Osteomyelitis microbiology
Radiography
Rats
Rats, Wistar
Saliva chemistry
Staphylococcal Infections microbiology
Tibia
Anti-Bacterial Agents therapeutic use
Biological Products therapeutic use
Erythromycin therapeutic use
Leeches chemistry
Methicillin-Resistant Staphylococcus aureus drug effects
Osteomyelitis drug therapy
Staphylococcal Infections drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 2567-6911
- Volume :
- 33
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Veterinary and comparative orthopaedics and traumatology : V.C.O.T
- Publication Type :
- Academic Journal
- Accession number :
- 32356297
- Full Text :
- https://doi.org/10.1055/s-0040-1703008