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The Vif protein of caprine arthritis encephalitis virus inhibits interferon production.
- Source :
-
Archives of virology [Arch Virol] 2020 Jul; Vol. 165 (7), pp. 1557-1567. Date of Electronic Publication: 2020 Apr 30. - Publication Year :
- 2020
-
Abstract
- Caprine arthritis-encephalitis (CAE) is a chronic progressive infectious disease caused by caprine arthritis-encephalitis virus (CAEV) that seriously threatens the goat industry. Chronic infection and life-long multi-tissue inflammation are the typical features of the disease. Innate antiviral immunity is essential for the host defense system that rapidly recognizes and eliminates invading viruses. Interferon β (IFN-β) is important for innate immunity and regulates immunity against a broad spectrum of viruses. To investigate the details of the IFN-β response to CAEV infection, the effects of six viral proteins and the molecular mechanisms by which they affect IFN-β production were analyzed. Overexpression of DU and Vif promote virus proliferation and inhibit the production of IFN-β. qRT-PCR and luciferase reporter assays showed that overexpression of Vif inhibits the expression of luciferase under the control of the ISRE, NF-κB or IFN-β promoter but does not affect the expression of IFN-β activated by IRF3, indicating that Vif negatively regulates IFN-β production by affecting upstream signal transduction of IRF3. Amino acids 149-164 of Vif were found to be necessary for the inhibitory effect of IFN-β production. Our results indicate that CAEV evades surveillance and clearance by intracellular innate immunity by downregulating IFN-β production.
- Subjects :
- Animals
Arthritis-Encephalitis Virus, Caprine genetics
Gene Products, vif genetics
Goat Diseases genetics
Goat Diseases virology
Goats
Host-Pathogen Interactions
Immunity, Innate
Interferon-beta genetics
Lentivirus Infections genetics
Lentivirus Infections immunology
Lentivirus Infections virology
NF-kappa B genetics
NF-kappa B immunology
Arthritis-Encephalitis Virus, Caprine immunology
Gene Products, vif immunology
Goat Diseases immunology
Interferon-beta immunology
Lentivirus Infections veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 165
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 32356187
- Full Text :
- https://doi.org/10.1007/s00705-020-04637-z