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Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
- Source :
-
PloS one [PLoS One] 2020 Apr 30; Vol. 15 (4), pp. e0232352. Date of Electronic Publication: 2020 Apr 30 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Cytokinin is an important regulator of symbiotic nodule development. Recently, KNOTTED1-LIKE HOMEOBOX 3 transcription factor (TF) was shown to regulate symbiotic nodule development possibly via the activation of cytokinin biosynthesis genes. However, the direct interaction between the KNOX3 TF and its target genes has not been investigated up to date. Here, using EMSA analysis and SPR-based assay, we found that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula. Moreover, we showed that MtLOG2 and MtIPT3 expression patterns partially overlap with MtKNOX3 expression in developing nodules as it was shown by promoter:GUS analysis. Our data suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Gene Expression Regulation, Developmental
Homeodomain Proteins genetics
Medicago truncatula growth & development
Plant Proteins genetics
Promoter Regions, Genetic
Transcription Factors genetics
Cytokinins biosynthesis
Gene Expression Regulation, Plant
Homeodomain Proteins metabolism
Medicago truncatula genetics
Plant Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 15
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 32353031
- Full Text :
- https://doi.org/10.1371/journal.pone.0232352