A Novel Glucose Isomerase from Caldicellulosiruptor bescii with Great Potentials in the Production of High-Fructose Corn Syrup.

Authors :: Dai C
Miao T
Hai J
Xiao Y
Li Y
Zhao J
Qiu H
Xu B
Source :: BioMed research international [Biomed Res Int] 2020 Apr 13; Vol. 2020, pp. 1871934. Date of Electronic Publication: 2020 Apr 13 (Print Publication: 2020).
Publication Year :: 2020
Abstract: Glucose isomerase (GI) that catalyzes the conversion of D-glucose to D-fructose is one of the most important industrial enzymes for the production of high-fructose corn syrup (HFCS). In this study, a novel GI (CbGI) was cloned from Caldicellulosiruptor bescii and expressed in Escherichia coli . The purified recombinant CbGI (rCbGI) showed neutral and thermophilic properties. It had optimal activities at pH 7.0 and 80°C and retained stability at 85°C. In comparison with other reported GIs, rCbGI exhibited higher substrate affinity ( K m = 42.61 mM) and greater conversion efficiency (up to 57.3% with 3M D-glucose as the substrate). The high catalytic efficiency and affinity of this CbGI is much valuable for the cost-effective production of HFCS.<br />Competing Interests: The authors declare no conflict of interest regarding the publication of this paper.<br /> (Copyright © 2020 Chenxia Dai et al.)

Subjects :: Aldose-Ketose Isomerases chemistry
Bacterial Proteins chemistry
Caldicellulosiruptor enzymology
High Fructose Corn Syrup chemistry
Zea mays chemistry

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