Back to Search
Start Over
Strict Preanalytical Oral Glucose Tolerance Test Blood Sample Handling Is Essential for Diagnosing Gestational Diabetes Mellitus.
- Source :
-
Diabetes care [Diabetes Care] 2020 Jul; Vol. 43 (7), pp. 1438-1441. Date of Electronic Publication: 2020 Apr 29. - Publication Year :
- 2020
-
Abstract
- Objective: Preanalytical processing of blood samples can affect plasma glucose measurement because ongoing glycolysis by cells prior to centrifugation can lower its concentration. In June 2017, ACT Pathology changed the processing of oral glucose tolerance test (OGTT) blood samples for pregnant women from a delayed to an early centrifugation protocol. The effect of this change on the rate of gestational diabetes mellitus (GDM) diagnosis was determined.<br />Research Design and Methods: All pregnant women in the Australian Capital Territory (ACT) are recommended for GDM testing with a 75-g OGTT using the World Health Organization diagnostic criteria. From January 2015 to May 2017, OGTT samples were collected into sodium fluoride (NaF) tubes and kept at room temperature until completion of the test (delayed centrifugation). From June 2017 to October 2018, OGTT samples in NaF tubes were centrifuged within 10 min (early centrifugation).<br />Results: A total of 7,509 women were tested with the delayed centrifugation protocol and 4,808 with the early centrifugation protocol. The mean glucose concentrations for the fasting, 1-h, and 2-h OGTT samples were, respectively, 0.24 mmol/L (5.4%), 0.34 mmol/L (4.9%), and 0.16 mmol/L (2.3%) higher using the early centrifugation protocol ( P < 0.0001 for all), increasing the GDM diagnosis rate from 11.6% ( n = 869/7,509) to 20.6% ( n = 1,007/4,887).<br />Conclusions: The findings of this study highlight the critical importance of the preanalytical processing protocol of OGTT blood samples used for diagnosing GDM. Delay in centrifuging of blood collected into NaF tubes will result in substantially lower rates of diagnosis than if blood is centrifuged early.<br /> (© 2020 by the American Diabetes Association.)
- Subjects :
- Adult
Australia
Blood Specimen Collection methods
Centrifugation standards
Clinical Laboratory Techniques methods
Clinical Laboratory Techniques standards
Diabetes, Gestational blood
Endocrinology methods
Endocrinology standards
False Positive Reactions
Fasting blood
Female
Glucose Tolerance Test methods
Glucose Tolerance Test standards
Humans
Pre-Analytical Phase methods
Pregnancy
Reproducibility of Results
Specimen Handling methods
Specimen Handling standards
Time Factors
Young Adult
Blood Glucose analysis
Blood Specimen Collection standards
Diabetes, Gestational diagnosis
Guideline Adherence standards
Pre-Analytical Phase standards
Subjects
Details
- Language :
- English
- ISSN :
- 1935-5548
- Volume :
- 43
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Diabetes care
- Publication Type :
- Academic Journal
- Accession number :
- 32350020
- Full Text :
- https://doi.org/10.2337/dc20-0304