Back to Search
Start Over
Remote ischemic conditioning protects against testicular ischemia∕reperfusion injury in rats.
- Source :
-
Acta cirurgica brasileira [Acta Cir Bras] 2020; Vol. 35 (2), pp. e202000203. Date of Electronic Publication: 2020 Apr 27. - Publication Year :
- 2020
-
Abstract
- Purpose To evaluate the effect of remote ischemic conditioning associated to N-acetylcysteine (NAC) on testicular ischemia∕reperfusion (I∕R) injury in rats. Methods Twenty-five adult male Wistar rats were randomly distributed into five experimental groups (n=5), as follows: Sham, I∕R, Perconditioning (PER), NAC and PER+NAC. Two-hour ischemia was induced by rotating the left testis 720° to clockwise direction, followed by 4 hours of reperfusion. Perconditioning was performed by three I/R cycles of 10 min each on the left limb, 30 min before reperfusion. N-acetylcysteine (150 mg∕kg) was administered 30 min before reperfusion. Results Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was statistical difference between PER and Sham, and PER+ NAC groups (p<0.05) in plasma. Conclusions The protective effect of perconditioning isolated in the reduction of lipid peroxidation related to oxidative stress was demonstrated. However, when Perconditioning was associated with NAC, there was no protective effect against testicular injury of ischemia and reperfusion.
- Subjects :
- Animals
Drug Evaluation, Preclinical
Male
Oxygen Radical Absorbance Capacity
Random Allocation
Rats
Rats, Wistar
Testis drug effects
Acetylcysteine pharmacology
Free Radical Scavengers pharmacology
Ischemic Preconditioning methods
Oxidative Stress drug effects
Reperfusion Injury
Testis blood supply
Subjects
Details
- Language :
- English
- ISSN :
- 1678-2674
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Acta cirurgica brasileira
- Publication Type :
- Academic Journal
- Accession number :
- 32348402
- Full Text :
- https://doi.org/10.1590/s0102-865020200020000003