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Total Internal Reflection Tip-Enhanced Raman Spectroscopy of Cytochrome c .
- Source :
-
The journal of physical chemistry letters [J Phys Chem Lett] 2020 May 21; Vol. 11 (10), pp. 3835-3840. Date of Electronic Publication: 2020 May 01. - Publication Year :
- 2020
-
Abstract
- Surface and tip-enhanced Raman spectroscopies in total internal reflection (TIR-SERS and TIR-TERS) are used to characterize the oxidation, spin, and ligation state of cytochrome c (Cc) molecules electrostatically bound on a hydrophilic thiol self-assembled monolayer. TIR-SERS spectra of this model hemoprotein show marker bands typical of the 6cLS ferric state of Cc. The performances of the TIR-TERS technique as a function of the incidence angle are described, showing in particular a significant electromagnetic enhancement of the Raman signal under p -polarized light excitation. TIR-TERS spectra of Cc confirm the 6cLS ferric state assignment deduced from TIR-SERS spectra, thus demonstrating the possibility of probing with nanoscale spatial resolution the 6cLS oxidized form of Cc that is potentially implicated in cell apoptotic processes. The minimal far-field contribution of the sample in TIR-TERS also offers promising perspectives for future nanoscale chemical characterizations of photosensitive biomolecules in complex biological media.
- Subjects :
- Spectrum Analysis, Raman
Surface Properties
Cytochromes c chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1948-7185
- Volume :
- 11
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The journal of physical chemistry letters
- Publication Type :
- Academic Journal
- Accession number :
- 32330046
- Full Text :
- https://doi.org/10.1021/acs.jpclett.0c00579