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Carboxylated Cellulose Nanocrystals Developed by Cu-Assisted H 2 O 2 Oxidation as Green Nanocarriers for Efficient Lysozyme Immobilization.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2020 May 27; Vol. 68 (21), pp. 5938-5950. Date of Electronic Publication: 2020 May 12. - Publication Year :
- 2020
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Abstract
- Cellulose nanocrystals (CNCs), having a high specific surface area and versatile surface chemistry, provide considerable potential to interact by various mechanisms with enzymes for nano-immobilization purposes. However, engineering chemically safe CNCs, suitable for edible administrations, presents a significant challenge. A reliable carboxylate form of H-CNCs was formed using H <subscript>2</subscript> O <subscript>2</subscript> oxidation of softwood pulp under mild thermal conditions. Negatively charged carboxyl groups (∼0.9 mmol g <superscript>-1</superscript> ) played a key role in lysozyme immobilization via electrostatic interactions and covalent linkages, as evidenced by Fourier transform infrared and <superscript>13</superscript> C cross-polarization magic angle spinning nuclear magnetic resonance spectroscopies. Adsorption isotherms showed a high loading capacity of H-CNCs (∼240 mg g <superscript>-1</superscript> ), and fitting the data to the Langmuir model confirmed monolayer coverage of lysozyme on their surface. Using a non-toxic coupling agent, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride, lysozyme-conjugated H-CNCs were developed with an immobilization yield of ∼65% and relative catalytic activity of ∼60%, similar to lysozyme adsorption on H-CNCs. These H-CNC-lysozyme nanohybrids, rationally processed via safe and green strategies, are specifically exploitable as catalytically active emulsifiers in food and pharmaceutical sectors.
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 68
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32330021
- Full Text :
- https://doi.org/10.1021/acs.jafc.0c00538